2 edition of Cyclic uptake of calcium-45 by dividing sea urchin eggs found in the catalog.
Cyclic uptake of calcium-45 by dividing sea urchin eggs
Galen Edward Clothier
Written in English
|Statement||by Galen Edward Clothier.|
|The Physical Object|
|Pagination||33 leaves, bound :|
|Number of Pages||33|
Skeletogenesis is a key morphogenetic event in the embryonic development of vertebrates and is of equal, although transient, importance in the development of the sea urchin, a marine invertebrate. The larval sea urchin does not resemble its adult form, because the sea urchin is an indirect developer, meaning its larva form must undergo metamorphosis to form the juvenile adult. Audio Books & Poetry Computers, Technology and Science Music, Arts & Culture News & Public Affairs Non-English Audio Spirituality & Religion Crônicas de um andarilho. Librivox Free Audiobook. Full text of "The Biological bulletin" See other formats. Journal of Economic Education Books by Language Additional Collections Journal of materials engineering. Journal of paediatric dentistry. Journal of Evolutionary Biochemistry and Physiology Journal of Experimental Psychology Full text of "The Echinoderms newsletter". In the early s, J. C. Dan (60, 61), studying the entrance of sea urchin sperm into eggs by phase-contrast and electron microscopy, discovered the acrosome reaction (AR). It is now established that this exocytotic process is one of the fundamental steps for fertilization in many species, including mammals (68, , , ).Cited by:
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Cyclic uptake of calcium by dividing sea urchin eggsAuthor: Galen Edward Clothier. Determination of calcium uptake and release The tracer calcium was added as "CaCIa to the ml suspension of fertilized eggs.
The amount of tracer was adjusted to give a final spec. act. of uCi/mg of total calcium (taking both the sea water calcium and the egg calcium into consideration).Cited by: Download PDF: Sorry, we are unable to provide the full text but you may find it at the following location(s): (external link).
CYCLIC UPTAKE OF CALCIUM BY DIVIDING SEA URCHIN EGGS INTRODUC ION The present studr was undertaken to provide direct data on the uptake of calcium by dividing eggs of the sea urchin. and to relate this, as far as possibles to specific mitotic events.
The role of calcium in cell division has been a subject of interest for many years the earlier ideas. Regular Article. The Cyclic GMP-Mediated Calcium Release Pathway in Sea Urchin Eggs Is Not Required for the Rise in Calcium during Fertilization. Abstract. The mechanisms required for cGMP-induced Ca 2+ release in the sea urchin egg were investigated using both egg homogenates and intact by: Ionophore A activations of the cortex of Lytechinus pictus and Strongylocentrotus purpuratus eggs were compared in various ionic media and were found to be completely independent of the ionic composition of the external by: In addition to acidic store alkalinization, it has been shown that fertilization of sea urchin eggs led to a change in cytosolic pH from to with a concomitant acidification of the sea water, and that the egg remains alkaline for approx.
60 min [48,49].Cited by: Fertilization or ionophore activation of Lytechinus pictus eggs can be monitored after injection with the Ca-sensitive photoprotein aequorin to estimate calcium release during activation.
We estimate the peak calcium transient to reach concentrations of – μM free calcium 45–60 sec after activation and to last 2–3 min, assuming equal Ca 2+ release throughout the by: Clothier G, Timourian H.
Calcium uptake and release by dividing sea urchin eggs. Exp Cell Res. ; – Epel D. The program of and mechanisms of fertilization in echinoderm egg. Amer Zool. ; – Ivy N, Rebhun LI. Calcium dependent activation of sea urchin eggs by sulfhydryl agents.
J Cell Biol. ; a. One of these monoclonal antibodies specifically labels punctate structures which appear in the mitotic apparatus of sea urchin eggs at the beginning of prophase and disappear upon the completion of cytokinesis. The antibody inhibits the Ca 2+ uptake of the membrane system in vitro.
It reacts with one kDa protein out of the complex protein Cited by: Intracellular calcium release at fertilization in the sea urchin egg* 1 We estimate the peak calcium transient to reach concentrations of & free calcium set after activation and.
Clothier (), using calciumobserved a regular uptake and release of radio - active calcium during the first mitotic cycle of sea urchin development. Three periods of uptake were noted and were related to mitotic events.
These periods were inter- preted as being associated with (1) protein gelation preceding the. Abstract. Graduation date: Developing sea urchin eggs (Strongylocentrotus purpuratus),\ud reared at 15° C, were subjected to single minute treatments with\ud disodium versenate in calcium-free sea water, applied at minute\ud intervals, from 4 minutes after insemination to the second cleavage.\ud These versene pulses produced specific degrees of retardation in\ud the mitotic process.
The first evidence for clues as to possible regulatory mechanisms of cADPR synthesis came from studies investigating the pathway for cGMP-induced Ca2+ mobilization in sea urchin eggs .
View. Sea urchin eggs have been an indispensable model system for studying egg activation and ionic signalling for at least a century. Instrumental in the discovery of two Ca 2+ -mobilizing second messengers, cyclic ADP-ribose and NAADP, the sea urchin has revolutionized cell biology for Cited by: Several lines of evidence in the literature indicate that environmental stress such as starvation may initiate reallocation of sea urchin endoskeletal tissue.
For example, Aristotle's lantern enlarges under conditions of starvation, and sea urchins tagged with tetracycline and then fed develop a distinct growth line, while starved individuals develop a diffuse by: Call Calcium () 18, Longman Group Ltd Calcium signals in and around the nucleus in sea urchin eggs I.
GILLOT and M. WHITAKER Department of Physiology, University College London, London, UK Abstract - A transient rise in cytoplasmic Ca 2+ activity in the sea urchin egg occurs during fertilization due to calcium release from an intracellular by: Galione A, Lee HC, Busa WB () Ca 2+-induced Ca 2+ release in sea urchin egg homogenates: modulation by cyclic ADP-ribose.
Science – CrossRef Google Scholar Cited by: 6. Of the three intracellular Ca 2+ signalling molecules (InsP 3, cADPR and NAADP) sea urchin egg homogenate has been used in the identification and characterisation of two, cADPR and nate is prepared in a Na + /Cl − substitute of N-Methyl glucamine (NMG)/ determine how media composition affects Ca 2+ release we replaced NMG with various sugars or Cited by: 3.
Abstract. The outer few microns of cytoplasm in eggs and embryos exert a powerful organizing influence during development. The cell cortex, as this “layer” is called, is a cellular domain whose dynamic properties account for much of the appearance and behavior of eggs and early by: 1 July Previous Issue; Next Issue; All Issues.
Cover Image. Cover Image. Experimental manipulation of the amount of tubulin available for assembly into the spindle of dividing sea urchin eggs.
G Sluder. View Article. Peroxidase uptake by photoreceptor terminals of the skate retina. H Ripps, M Shakib, E D MacDonald. View Article. NAADP-induced calcium release in sea urchin eggs Article Literature Review in Biology of the Cell 92() August with 71 Reads How we measure 'reads'.
Developing sea urchin eggs (Strongylocentrotus purpuratus), reared at 15° C, were subjected to single minute treatments with disodium versenate in calcium-free sea water, applied at minute intervals, from 4 minutes after insemination to the second by: 4.
Sea urchin eggs and those of most other deuterostomes are activated at fertilization by an increase in cytoplasmic free calcium concentration ([Ca 2+ ]i) that triggers the onset of the embryonic.
The process of secretory granule-plasma membrane fusion can be studied in sea urchin eggs. Micromolar calcium concentrations are all that is required to bring about exocytosisin vitro.
I discuss recent experiments with sea urchin eggs that concentrate on the biophysical aspects of granule-membrane fusion.
The backbone of biological membranes is the lipid by: Bio Ch47 Self Quiz. STUDY. Flashcards. Learn. Write. Spell. Test. PLAY. Match. Gravity. Created by. adfsfsfsf. Terms in this set (10) The cortical reaction of sea urchin eggs functions directly in the.
formation of a fertilization envelope. Which of the following is common to both avian and mammalian development. epiblast and hypoblast. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): ABSTRACT We isolated calcium-labile mitotic spindles from eggs of the sea urchin Lytechinus variegatus, using a low ionic strength, EGTA lysis buffer that contained mM EGTA, mM M9Cl2, mM PIPES, pHwith 1 % Nonidet P (detergent) and % glycerol.
Calcium Inﬂux Mediates the Voltage-Dependence of Sperm Entry into Sea Urchin Eggs David H. McCulloh,1 Pedro I. Ivonnet, David Landowne, and Edward L. Chambers Department of Physiology and Biophysics, University of Miami School of Medicine, P.O.
BoxMiami, Florida - Internal fertilization, heterogeneity of sperm population, transport of both games to the oviduct with translocation of gametes, sperm capacitation, thermotaxis, hyper-activation of motility, and recognition at the zona pellucida (vitelline envelope in urchin eggs) are the differences from the sea urchin.
Exposure of sea urchin eggs and sperm to these effluents will require adjustments in the salinity of the test solutions. It is important to maintain a constant salinity across all treatments. In addition it may be desirable to match the test salinity with that of the receiving water. Two methods are available to.
Evidence is steadily mounting that the proto-oncogenes, whose products organize and start the programs that drive normal eukaryotic cells through their chromosome replication/mitosis cycles, are transiently stimulated by sequential signals from a multi-purpose, receptor-operated mechanism (consisting of internal surges of Ca2+ and bursts of protein kinase C activity resulting from Cited by: This resulted in increased MAP kinase phosphatase activity.
Therefore, MAP kinase inactivation at fertilization in sea urchin eggs likely is the result of a combination of MEK inactivation and phosphatase activation that are directly or indirectly responsive to Ca(2+). DOI: /dbio Alternate Journal: Dev. Biol. PubMed ID: uptake during the first mitotic division in sea urchin eggs.
A definite relationship was shown to exist between the periods of uptake and specific mitotic events. The first peak of calcium 45 uptake occurred at mid-prophase and was related to gelation of spindle precursor.
The second uptake, occurring at mid-metaphase, was related to gelation of interzonal spindle precursor. The third peak. Unlike the eggs of most animals, sea urchin eggs have completed meiosis prior to fertilization and are arrested at the pronuclear stage.
Using both phosphorylation-state-specific antibodies and a MAP kinase activity assay, we observe that MAP kinase is phosphorylated and active in unfertilized sea urchin eggs and then dephosphorylated and. The Effects of Calcium-Free Seawater on the Development of Sea Urchin Embryos Amy Yanega '01 Objective The purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos.
Embryos were placed in calcium-free sea after fertilization and at the initiation of gastrulation. Nath J, Rebhun LI.
Studies on cyclic AMP phosphodiesterase in sea urchin eggs. Biochim Biophys Acta. Dec 29; (2)– Okazaki Y, Mabuchi I, Kimura I, Sakai H. Binding sites of -SH reagents in dividing sea urchin egg. Exp Cell Res. Dec; 82 (2)– Turner PR, Jaffe LA, Primakoff P.
A cholera toxin-sensitive G-protein stimulates exocytosis in sea urchin eggs. Dev Biol. Apr; (2)– Swann K, Whitaker M. The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchin eggs. J Cell Biol. Dec; (6 Pt 1)– the sea urchin is already a haploid by the time of fertilization, and in mammals the egg is a diploid until the sperm enters and the eggs meiotic divisions are completed after, in what stage of meiosis is the human egg when it binds to sperm.
The NAD+ metabolite cADP-Rib (cADPR) elevates cytosolic free Ca2+ in plants and thereby plays a central role in signal transduction pathways evoked by the drought and stress hormone abscisic acid.
cADPR is known to mobilize Ca2+ from the large vacuole of mature cells. To determine whether additional sites for cADPR-gated Ca2+ release reside in plant cells, microsomes from Cited by: The Calcium Transient in Sea Urchin Eggs during Fertilization Requires the Production of Inositol 1,4,5-Trisphosphate1 Shyh-Jye Lee and Sheldon S.
Shen Department of Zoology & Genetics, Iowa State University, Ames, Iowa The production of inositol 1,4,5-trisphosphate (InsP 3) has been reported to mediate the transient rise in. Lab Report 2: The Role of the Cytoplasmic Calcium Influx during Sea Urchin Fertilization.
(Labs ) Always refer to the general lab report guidelines for information on how to format your report.
As always, all the labs in this project are written up as a single lab report. How to approach this lab report: The report should be written as a scientific research article in which you are.Start studying BIO study.
Learn vocabulary, terms, and more with flashcards, games, and other study tools. an organ that connects the developing fetus to the uterine wall to allow a nutrient uptake. when the head of a sea urchin sperm contacts the jelly coat of a sea urchin egg, molecules in the eggs coat trigger the _____ acrosome.Start studying Development 1.
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